Cell counting is a recurring bottleneck in high-throughput cell-culture workflows. Existing options can be hard to automate, limited in throughput, or expensive because of the instruments and consumables they require. Here we show our novel microfluidic plates and the CellOpsis Bench system used for high-throughput cell counting.
Example Images From the Microfluidic Plate
Slide to reveal the cell mask over a brightfield image from the microfluidic plate.
Mask
Raw
Approach
A fixed volume of CHO DG44 cells was diluted to 100%, 75%, 50%, and 25% of the original suspension. Three 10 uL samples from each dilution were loaded into a chamber in the VolkCell microfluidic plate and imaged with CellOpsis Bench using a 4x brightfield objective.
- Readout: observed cell count
- Replicates: n=3 per concentration
- Analysis: VolkCell image classification model
Results
The observed cell count followed the expected dilution response, with a four-point linear regression fit of R² 0.927. Across the dilution series, coefficients of variation ranged from 6.0% to 13.6%.
- 1.00x: mean 2114.3, CV 8.8%
- 0.75x: mean 1195.0, CV 13.6%
- 0.50x: mean 989.0, CV 6.0%
- 0.25x: mean 517.3, CV 12.8%
Request the application note
The full application note includes the experiment summary, image-analysis example, dilution-series chart, and statistics table for the automated cell-counting workflow.
Request application note →